Wang XY[1][2], Chu ZS[2][3], Zhang H[1], Zhang SQ[4], Pan SY[2], Tang JF[1], Ko KM[5],
[1] Department of Parmacy, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, China.
[2] School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
[3] Student Affairs Department, Zhejiang Chinese Medical University, Hangzhou, China
[4] School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
[5] Division of Life Science, The Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong SAR, China.
. Aim: To evaluate the mouse model of hypertriglyceridemia (hTG) induced by schisandrin B (Sch B) using lipid metabolomics technology. Methods: Male ICR mice weighing 23 ~ 27 g were randomly divided into four groups: (1) mice fed with normal diet (ND group) (2) mice fed with ND and treated with Sch B(ND +Sch B group); (3) mice fed with high fat/fructose diet(HFFD group; fat, 10%; fructose, 10%; w/w), and (4) mice fed with HFFD and treated with Sch B (HFFD + Sch B group). Based on our previous research, Sch B at a single dose of 2 g ¡P kg-1 was orally administered to the animals in the current study. Forty-eight hours later, serum samples were obtained from the orbital vein. Serum triglyceride (TG) and total cholesterol (TC) were analyzed by biochemical method. The metabolic fingerprint spectrum of serum in all groups were obtained and analyzed using ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q/TOF-MS) method. The differences of metabolic spectra in every two groups were compared via the multivariate statistical methods. Results: Compared with ND group, three kinds (27 markers) of differential metabolites were identified in ND +Sch B group, including 18 TG, 7 phosphatidylcholine (PC), and 2 phosphatidylethano-lamine(PE). Compared with ND group, five kinds(27 markers) of differential metabolites were screened in HFFD group, including 6 sphingomyelin (SM), 13 PC, 2 cholesteryl ester(CE), 5 TG and 1 phosphati-dylinositol (PI). Compared with HFFD group, four kinds (25 markers) of differential metabolites were found in HFFD + Sch B group, involving 14 TG, 1 CE, 6 PC and 4 PE. Conclusion: These findings suggest that the animal model of hypertriglyceridemia established by Sch B involves the alteration of serum lipid metabolomics.